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Table 1 The loading capacity and extraction recovery of single-StageTip microcolumns

From: A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

 

Recovery of3 H-labelled CKs (%)

 

C18

SDB-RPS

Cation-SR

 

Flow through

Elution

Flow through

Elution

Flow through

Elution

[3H]c Z

98.8 ± 3.3

0.6 ± 0.2

1.3 ± 0.1

96.6 ± 1.8

1.1 ± 0.1

100.7 ± 3.0

[3H]t ZR

98.8 ± 4.4

0.6 ± 0.2

39.2 ± 2.2

29.8 ± 2.2

22.4 ± 0.6

64.7 ± 1.1

[3H]iPR

102.9 ± 3.5

0.5 ± 0.3

4.9 ± 0.2

95.6 ± 1.4

4.7 ± 0.1

97.0 ± 2.8

  1. Values are means ± SD (n = 4).
  2. Recovery (%) of 3 H-labelled CK standards without added plant matrix. Tritium-labelled standards (105 dpm) were dissolved in Bieleski buffer and applied onto different Empore sorbents (C18, SDB-RPS, Cation-SR).
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Plant Methods

ISSN: 1746-4811