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Table 1 The loading capacity and extraction recovery of single-StageTip microcolumns

From: A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

  Recovery of3 H-labelled CKs (%)
  C18 SDB-RPS Cation-SR
  Flow through Elution Flow through Elution Flow through Elution
[3H]c Z 98.8 ± 3.3 0.6 ± 0.2 1.3 ± 0.1 96.6 ± 1.8 1.1 ± 0.1 100.7 ± 3.0
[3H]t ZR 98.8 ± 4.4 0.6 ± 0.2 39.2 ± 2.2 29.8 ± 2.2 22.4 ± 0.6 64.7 ± 1.1
[3H]iPR 102.9 ± 3.5 0.5 ± 0.3 4.9 ± 0.2 95.6 ± 1.4 4.7 ± 0.1 97.0 ± 2.8
  1. Values are means ± SD (n = 4).
  2. Recovery (%) of 3 H-labelled CK standards without added plant matrix. Tritium-labelled standards (105 dpm) were dissolved in Bieleski buffer and applied onto different Empore sorbents (C18, SDB-RPS, Cation-SR).
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