|
Recovery of3 H-labelled CKs (%)
|
---|
|
C18
|
SDB-RPS
|
Cation-SR
|
---|
|
Flow through
|
Elution
|
Flow through
|
Elution
|
Flow through
|
Elution
|
---|
[3H]c Z
|
98.8 ± 3.3
|
0.6 ± 0.2
|
1.3 ± 0.1
|
96.6 ± 1.8
|
1.1 ± 0.1
|
100.7 ± 3.0
|
[3H]t ZR
|
98.8 ± 4.4
|
0.6 ± 0.2
|
39.2 ± 2.2
|
29.8 ± 2.2
|
22.4 ± 0.6
|
64.7 ± 1.1
|
[3H]iPR
|
102.9 ± 3.5
|
0.5 ± 0.3
|
4.9 ± 0.2
|
95.6 ± 1.4
|
4.7 ± 0.1
|
97.0 ± 2.8
|
- Values are means ± SD (n = 4).
- Recovery (%) of 3 H-labelled CK standards without added plant matrix. Tritium-labelled standards (105 dpm) were dissolved in Bieleski buffer and applied onto different Empore sorbents (C18, SDB-RPS, Cation-SR).