Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Table 4 Effect of pre-treatment with opening buffer (OB) on the ‘rundown’ of I _K,in and I _K,out in Arabidopsis guard cells of Col-0, nia1nia2 and QC3

Channel	Current (μA cm^-2)	Col-0		*nia1nia2*		*QC3*
		10 min	30 min	10 min	30 min	10 min	30 min
I_K,in	Control	-484.1 ±185.6	-6.43 ±3.3**	-118.7±30.2	-1.2±10.3**	-413.8±142.1	-8.1±0.3**
	Pretreatment	-586.3 ±40.7	-431.9±75.2	-223.0 ±49.5	-247.1±54.8	-602.1±112.4	-543.2±44.3
I_K,out	Control	598.2 ±89.0	145.29±36.0**	527.9±90.1	71.2±10.4**	591.2±111.1	168.4±119.5**
	Prereatment	901.0 ±271.5	921.64 ±236.4	698.8 ±147.2	669.9±174.9	741.3±198.8	732.3±127.0

Data are means ± SE from the analysis of >5 experiments extending over 60 min or more in each case.
**P < 0.01 as compared with control.

ISSN: 1746-4811