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Table 4 Effect of pre-treatment with opening buffer (OB) on the ‘rundown’ of I K,in and I K,out in Arabidopsis guard cells of Col-0, nia1nia2 and QC3

From: Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Channel Current (μA cm-2) Col-0   nia1nia2   QC3  
   10 min 30 min 10 min 30 min 10 min 30 min
IK,in Control -484.1 ±185.6 -6.43 ±3.3** -118.7±30.2 -1.2±10.3** -413.8±142.1 -8.1±0.3**
  Pretreatment -586.3 ±40.7 -431.9±75.2 -223.0 ±49.5 -247.1±54.8 -602.1±112.4 -543.2±44.3
IK,out Control 598.2 ±89.0 145.29±36.0** 527.9±90.1 71.2±10.4** 591.2±111.1 168.4±119.5**
  Prereatment 901.0 ±271.5 921.64 ±236.4 698.8 ±147.2 669.9±174.9 741.3±198.8 732.3±127.0
  1. Data are means ± SE from the analysis of >5 experiments extending over 60 min or more in each case.
  2. **P < 0.01 as compared with control.