Table 3 Analysis of maximal conductance (g max ), gating charge (δ), half maximal voltage (V 1/2 ) of I K,in and I K,out for the Arabidopsis lines Col-0, nia1nia2 , QC3, QL3, kc1-3, syp121, syp121ox, and dhar1-3 in both opening and non-condition
From: Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis
Channel | Parameters | Average of 8 lines | Col-0 | Â | nia1nia2 | Â | QC3 | Â | |
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Control | Pretreatment | Control | Pretreatment | Control | Pretreatment | Control | Pretreatment |
IK,in | gmax (mS cm-2) | 2.3±0.1 (103) | 2.0±0.2 (67) | 2.2±0.2 (26) | 2.6±0.2* (15) | 0.3±0.1 (10) | 0.9±0.1** (36) | 3.4±0.3 (21) | 3.2±0.2 (13) |
|  | δ | 2.2±0.1 (103) | 1.9±0.1* (67) | 2.4±0.1 (26) | 2.0±0.1* (15) | 1.1±0.2 (10) | 1.9±0.1** (36) | 2.1±0.2 (21) | 1.9±0.2 (13) |
|  | V1/2 (mV) | -186±2.1 (103) | -182±1.6 (67) | -185±3 (26) | -182±2 (15) | -180±10 (10) | -179±2.5 (36) | -185±4.3 (21) | 188±2.6 (13) |
IK,out | gmax (mS cm-2) | 3.0±0.1 (103) | 3.5±0.1* (67) | 2.6±0.2 (26) | 3.8±0.6** (15) | 2.7±0.3 (10) | 3.6±0.2* (36) | 3.9±0.3 (21) | 3.5±0.3 (13) |
|  | δ | 1.6±0.1 (103) | 1.6±0.1 (67) | 1.8±0.1 (26) | 1.6±0.1 (15) | 1.5±0.1 (10) | 1.5±0.1 (36) | 1.5±0.2 (21) | 1.5±0.2 (13) |
|  | V1/2 (mV) | 0.9±1.7 (103) | -3.6±1.7 (67) | 6.0±2.6 (26) | 0.4±2.3 (15) | -10±4.6 (10) | -2.4±2.4 (36) | -4.1±4.1 (21) | -6.5±5.7 (13) |