Effects of genistein on mRNA level of a transcriptionally silenced endogenous gene in
plants. Real-time RT-PCR was conducted to analyze mRNA level of TSI gene in Arabidopsis plants treated with genistein (20 μM or 50 μM) and 5-azaC (20 μM). The mRNA levels were quantified relative to the mRNA level of eIF4A1. The value of control plants grown in a medium with no supplement ('Media alone') was set at 1. Data represent the means and standard errors obtained from three replicates. Statistic analysis using Student's t-test indicated that the mRNA levels of TSI gene in plants treated with these compounds were significantly higher than those of non-treated plants (P < 0.05).