Effects of genistein on reactivation of the transcriptionally silenced
transgene in petunia plants. Real-time RT-PCR was conducted to analyze the mRNA levels of the CHS-A transgene in plants treated with genistein (50 μM), 5-azaC (20 μM) and TSA (6 μM). The mRNA levels were quantified relative to the mRNA level of α-tubulin. The value for control plants grown in a medium with no supplement ('Media alone') was set at 1. Data represent means and standard errors obtained from three replicates. Statistic analysis using Student's t-test indicated that the mRNA levels of CHS-A transgene in plants treated with these compounds were significantly higher than those of non-treated plants (P < 0.05).