Figure 4
From: Precursor uptake assays and metabolic analyses in isolated tomato fruit chromoplasts
Analysis of metabolic intermediates in isolated tomato fruit chromoplasts. Isolated chomoplasts were incubated with [14C]-pyruvate (A) and [14C]-malate (B) for 30, 60 and 120 min. At the end of the incubation periods water soluble compounds were analyzed by HPLC (Aminex HPX-87H column) coupled to a radioactivity detector. Identified compounds are indicated: 1) pyruvate, 2) acetate and 3) malate. Results displayed are representative of at least three different experiments.