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Figure 3 | Plant Methods

Figure 3

From: Precursor uptake assays and metabolic analyses in isolated tomato fruit chromoplasts

Figure 3

Analysis of radiolabeled compounds in isolated tomato fruit chromoplasts. Isolated chromoplasts were incubated with [14C]-pyruvate for 24 h and then extracted with hexane:acetone:methanol (2:1:1). Labeled compounds present in the organic fraction were separated by TLC and detected by autoradiography (A). Isolated chromoplasts were incubated with [14C]-pyruvate in the absence or presence of glyceraldehyde 3-phosphate (GAP) for 24 h and extracted with hexane:acetone:methanol (2:1:1). Labeled compounds present in the organic fraction were separated by TLC and detected by autoradiography (B). Eluents for TLC were hexane:ether:acetone (60:30:20) (A) and chloroform:methanol:water (65:25:4) (B). Results displayed are representative of at least three different experiments.

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