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Figure 6 | Plant Methods

Figure 6

From: Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis

Figure 6

Real time qRT-PCR analysis of FLC expression on plant samples harvested after four-weeks cold treatment and extracted using either the P:C-L or HTP96 methods. The pattern of FLC expression was compared using RNA extracted from both the P:C-L and HTP96 methods. Plant samples from two different accessions were harvested without cold treatment (NV), and zero (4V0), ten (4V10) or 30 days (4V30) after a four-week cold treatment. Total RNA was extracted using either the P:C-L or HTP96 methods. Relative FLC expression of two biological replicates was assayed by SYBR green real time qRT-PCR, each consisting of three technical replicates. Error bars represent the standard error of the mean. A. FLC expression in Col FRI-SF2. B. FLC expression in Löv-1.

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