Figure 5
From: Development of expression vectors based on pepino mosaic virus
PepMV vector including a GFP:CP fusion separated by the FMDV 2A catalytic peptide sequence. (a) Schematic representation of PepGFP2a. The position of the 2A sequence insertion is indicated. (b) Fluorescence in systemically infected N. benthamiana leaves from plants agroinfiltrated with pBPepGFP2a. (c) Northern blot of leaf tissue hybridized with a CP-specific RNA probe to determine PepGFP2a stability. Total RNA was prepared from agroinfiltrated and systemically-infected leaves showing fluorescence at 7 and 14 dpi, and from plants mechanically inoculated with PepGFP2a at 7 dpi after the second and third passages. The band corresponding to the GFP-CP sgRNA is marked with star. Ethidium bromide-stained rRNA is shown (bottom panels). (d) GFP expression after second and third passage of PepGFP2a. Plants were photographed under white and UV light at 7 and 12 dpi.