Figure 3
From: Development of expression vectors based on pepino mosaic virus
PepMV vectors containing a duplicated subgenomic mRNA promoter. (a) In Pep5.128, the GFP gene was positioned downstream of the CP gene, whereas it was positioned upstream of the CP gene in Pep501. The duplicated SGP spanned positions -76 to +36 relative to the CP start codon. Pep352 and Pep640 include successive deletions at the 3' end of the putative CP SGP. SGP-CP subgenomic mRNA promoter; SGPd-duplicated CP subgenomic mRNA promoter. (b) Green fluorescence visible in systemically-infected N. benthamiana leaves from plants agroinfiltrated with pBPep501 at 5 and 7 dpi. (c) Northern blot of leaf tissue hybridized with a CP-specific RNA probe to determine Pep501 stability. Total RNA was prepared from systemically-infected leaves at 5 and 7 dpi. The band corresponding to the GFP-CP sgRNA is marked with star. Ethidium bromide-stained rRNA is shown (bottom panels). (d) N. benthamiana leaves infiltrated with pBPep352 or pBPep501 vectors, showing fluorescence emitted under UV illumination with a handheld UV lamp. Photographs were taken at 5 or 7 dpi.