Figure 5

Confirmation of transgenic status of the regenerated rice plants. GUS expression in leaves (a-d); PCR amplification of transgene from genomic DNA (e-h) and Southern analysis (i-l) of rice cultivars IR64, PB1, CSR10 and Swarna respectively. For Southern analysis, genomic DNA was restricted with a single cutter enzyme (Xba I) of T-DNA to indicate the copy number of the transgene and independence of the events. (m) Regeneration frequency (%)^p of untransformed calli (regenerated without co-cultivation with Agrobacterium) and transformed calli using either LBA4404 or EHA105. (n) Transformation efficiency (%)^q of IR64, PB1, CSR10 and Swarna using different Agrobacterium strains viz. LBA4404 or EHA105. Error bars, standard deviations; n = 3. ^pRegeneration Frequency (%) = no. of microcalli regenerating shoots/no. of microcalli incubated × 100%. ^qTransformation efficiency (%) = no. of plants expressing GUS/no. of calli inoculated with Agrobacterium X 100%