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Table 1 Identification of proteins assigned to the apoplast.

From: Comparative evaluation of extraction methods for apoplastic proteins from maize leaves

    

Infiltration solutions

Entry

Description

mW (Da)

pI (pH)

i

ii

iii

iv

v

vi

Cell growth and division

        

UniRef90_Q6ZDE3

Abscisic acid 8'-hydroxylase 2, Oryza sativa

56519

9.83

x

     

Cell structure

        

UniRef90_A5H454

Peroxidase 66, Zea maize

33398

8.02

    

x

 

UniRef90_A5H8G4

Peroxidase 1, Zea maize

38330

6.89

    

x

 

UniRef90_A5JTQ2

Alpha N arabinofuranosidase, Medicago varia

83673

6.22

 

x

    

UniRef90_B4FKV6

Peroxidase 54, Zea maize

36178

4.95

     

x

UniRef90_B5AK47

Dhurrinase-like B glucosidase, Zea maize

64233

7.95

  

x

   

UniRef90_B6SMR2

Peroxidase 52, Zea mays

33504

8.14

 

x

x

 

x

x

UniRef90_B6SUH6

Non-cyanogenic beta glucosidase, Zea mays

56680

5.39

x

 

x

 

x

 

UniRef90_B6SXU7

Heparanase-like protein 3, Zea maize

58262

9.35

x

 

x

x

x

x

UniRef90_B6SXY3

Beta galactosidase, Zea mays

48728

8.38

 

x

    

UniRef90_B6T391

Lichenase 2, Zea mays

34951

5.64

x

x

x

x

x

x

UniRef90_B6T9B9

Alpha N arabinofuranosidase, Zea mays

74827

5.04

  

x

 

x

x

UniRef90_B6TU39

Peroxidase 2, Zea maize

34941

4.67

 

x

    

UniRef90_B6TU78

Glucan endo-1-3 beta glucosidase 7, Zea maize

45316

5.54

x

     

UniRef90_B6TXJ8

Glycoside hydrolase family 28, Zea maize

47066

5.58

x

     

UniRef90_B6U063

Carboxylic ester hydrolase, Zea mays

50032

7.85

x

 

x

 

x

 

UniRef90_B6U0W2

Beta galactosidase, Zea maize

93935

6.48

 

x

    

UniRef90_B9SD68

Hydrolase, Ricinus communis

67825

6.80

x

x

x

   

UniRef90_C4N559

Xyloglucan endotransglycosylase hydrolase, Musa acuminata

20309

9.54

     

x

UniRef90_C5WQU7

Beta galactosidase, Sorghum bicolor

92893

5.31

x

 

x

x

x

x

UniRef90_C5Z534

Beta galactosidase, Sorghum bicolor

79098

7.74

 

x

    

UniRef90_O04943

Alpha galactosidase, Hordeum vulgare

17730

6.33

 

x

x

 

x

x

UniRef90_P93518

PRm 3, Zea maize

30099

3.90

x

x

x

x

x

x

UniRef90_Q10CU3

Glycosyl hydrolase family 3, Oryza sativa

43916

8.20

x

 

x

   

UniRef90_Q10M79

Alpha L arabinofuranosidase, Oryza sativa

73965

4.73

    

x

 

UniRef90_Q10NX8

Beta galactosidase 6, Oryza sativa

92780

5.52

x

  

x

  

UniRef90_Q2R3E0

Glycosyl hydrolases family 38, Oryza sativa

114085

5.85

 

x

   

x

UniRef90_Q2RAZ2

Alpha L arabinofuranosidase, Oryza sativa

73421

4.57

x

 

x

x

x

x

UniRef90_Q43417

Peroxidase, Cenchrus ciliaris

32473

7.50

    

x

 

UniRef90_Q53MP2

Beta D-xylosidase, Oryza sativa

82557

6.62

 

x

    

UniRef90_Q5CCP6

Beta galactosidase, Pyrus pyrifolia

94782

8.12

 

x

    

UniRef90_Q5I3F3

Peroxidase 5, Triticum monococcum

27533

5.72

 

x

    

UniRef90_Q6L619

Beta galactosidase, Raphanus sativus

92580

8.36

x

   

x

 

UniRef90_Q7G3T8

Beta galactosidase 13, Oryza sativa

91940

6.06

 

x

    

UniRef90_Q8GUY1

Pectinesterase, Lolium perenne

24837

7.81

 

x

    

UniRef90_Q8RUV9

Beta galactosidase 1, Oryza sativa

91652

5.71

 

x

    

UniRef90_Q9FXT4

Alpha galactosidase, Oryza sativa

45792

7.91

    

x

x

UniRef90_Q9LLB8

Exoglucanase, Zea mays

66900

6.99

x

x

x

x

 

x

UniRef90_Q9XE93

Exhydrolase II, Zea mays

68330

6.16

x

x

x

x

x

x

UniRef90_Q9XEI3

Beta D-glucan exohydrolase isoenzyme, Hordeum vulgare

67862

6.24

x

x

 

x

  

Disease and defence

        

UniRef100_P25272

Kunitz-type trypsin inhibitor 1, Glycine max

22531

4.77

x

     

UniRef90_A7IZL3

Invertase inhibitor, Coffea canephora

20205

6.68

 

x

    

UniRef90_B6TA80

Thaumatin-like protein, Zea mays

17632

6.75

    

x

 

UniRef90_B6TDW7

Secretory protein, Zea mays

24467

4.64

    

x

 

UniRef90_B6TT00

Endochitinase PR4, Zea maize

28545

4.96

x

   

x

 

UniRef90_B6TTY1

Germin-like protein, Zea maize

26764

7.25

x

 

x

 

x

x

UniRef90_B6TWH6

Lysosomal Pro × carboxypeptidase, Zea mays

59936

5.62

x

x

x

x

 

x

UniRef90_B6UB57

Lysosomal protective protein, Zea maize

53540

5.87

x

x

x

 

x

x

UniRef90_O24007

Chitinase, Oryza sativa

18956

4.83

x

 

x

x

x

x

UniRef90_P01063

Bowman-Birk-type proteinase inhibitor C II, Glycine max

9194

4.38

x

     

UniRef90_P29022

Endochitinase A, Zea maize

29105

7.85

    

x

 

UniRef90_Q5U1S9

Class III peroxidase 14, Oryza sativa

37174

5.77

    

x

 

UniRef90_Q6EUS1

Class III peroxidase 27, Oryza sativa

33300

8.09

 

x

   

x

UniRef90_Q6TM44

Germin-like protein, Zea mays

21873

6.04

x

x

x

x

x

x

UniRef90_Q7M1R1

Chitinase, Gladiolus × gandavensis

30695

5.77

x

 

x

x

x

x

Protein destination and storage

        

UniRef90_B6TG95

Vignain, Zea mays

38823

4.68

x

x

x

x

x

x

UniRef90_B6TYX7

Polygalacturonase inhibitor 1, Zea mays

30011

8.08

 

x

  

x

x

Secondary metabolism

        

UniRef90_O64411

Polyamine oxidase, Zea mays

56308

5.63

 

x

    

Signal transduction

        

UniRef90_B6TWC3

Rhicadhesin receptor, Zea mays

23726

9.20

 

x

x

   

UniRef90_B9MZ47

Fasciclin-like AGP 14 4 protein, Populus trichocarpa

24786

8.63

    

x

 

Transporters

        

UniRef90_B4FB54

Non-specific lipid transfer protein, Zea mays

12084

9.60

 

x

    

UniRef90_B6SP11

Non-specific lipid transfer protein, Zea mays

9802

8.73

 

x

    

UniRef90_B6SY96

Non-specific lipid transfer protein, Zea mays

12011

9.29

 

x

    

UniRef90_B6TRB2

Copper ion binding protein, Zea maize

17057

9.78

  

x

   

UniRef90_P05046

Lectin, Glycine max

30908

5.60

x

  

x

  

UniRef90_P19656

Non-specific lipid transfer protein, Zea mays

11697

8.74

 

x

   

x

UniRef90_Q04672

Sucrose-binding protein, Glycine max

60484

6.42

x

     
  1. UniProt database identifiers, along with molecular weight (mW) and isoelectric point (pI) are shown. The identification of the respective proteins using different extraction solutions is indicated (i: water, ii: 100 mM sodium phosphate buffer, iii: 25 mM Tris-HCl, iv: 100 mM sorbitol, v: 20 mM ascorbic acid/20 mM CaCl2, vi: 50 mM NaCl).