Figure 2
From: Comparative evaluation of extraction methods for apoplastic proteins from maize leaves
Profiles of maize leaf protein extracts as resolved by 1-DE (A) and 2-DE (B). A: SDS-PAGE of apoplastic and symplastic proteins extracted with water (i), 100 mM sodium phosphate buffer (ii), 25 mM Tris-HCl (iii), 100 mM sorbitol (iv), 20 mM ascorbic acid/20 mM CaCl2 (v) or 50 mM NaCl (vi). A total of 10 μg protein per lane was loaded. B: 2-DE profiles of protein extracts from the maize leaf apoplast isoelectric focussed on IPG 3-10 and visualized by Coomassie staining. A total of 25 μg protein per gel was loaded.