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Figure 6 | Plant Methods

Figure 6

From: Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of pea

Figure 6

Microscopy of spot-inoculated lateral roots. A. An unstained longitudinal section of a spot-inoculated lateral root 24 hours after rhizobial inoculation showing anticlinal divisions in its inner cortex (arrows), and in its pericycle (asterisk), located adjacent to a xylem pole (X). Bar: 100 μm. B. A close-up on the area framed in Figure A. Metabolically active cells, one with a large nucleus (arrowhead), exhibit anticlinal divisions. Bar: 10 μm. C. A longitudinal section of a root inoculated 3 days earlier with rhizobia and stained with toluidine blue. The nodule meristem (NM) has passed the mid-point of the root cortical region. Bar: 100 μm. Note that for all the photographs, there was no indication of nodule emergence and it was only because of the spot-inoculation that these events were easily captured. All sections were made with a vibratome.

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