Figure 5

Macro-photography of nodules developing on roots of plants grown in pouches. A. An overview of an 8 day-old spot-inoculated plant. B. A close-up focussed on the root hairs of the seedling 5 DAI. The unfocussed black dots are located on the surface of the pouch and correspond to the different inoculation sites. In C to F, two nodules (arrowheads) can be seen developing over time. The nodules are five-day old (C), seven-day old (D), 10 day-old (E) and 14 day-old (F). In E and F, two additional nodules (black circles) appeared later at the spot-inoculation site; they cannot be given a specific age. One (asterisks in F) of the nodules appears much larger than the others; it is evident that this large nodule has multiple meristems. In D, two roots (arrows) which did not grow in contact with the filter paper of the pouch dried out. White arrowheads indicate pieces of vermiculite.