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Figure 2 | Plant Methods

Figure 2

From: Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of pea

Figure 2

Illustrations depicting the main steps for the production of composite pea plants. A. Plants grown for 10 days in vermiculite (7 days in dark followed by 3 in light). B. Fibrgro® cubes saturated with A. rhizogenes. C. A shoot of a 10 day-old plant cut above node 2 and inserted into Fibrgro® cube. D. A wilted pea plant about to receive water for recovery. E. Plants placed in growth tray under a plastic dome. F. High humidity is provided by plastic lid with closed ventilation holes and metal tray with water. G. A root (arrow) protruding through a Fibrgro® cube 10 days after A. rhizogenes infection. H. Callus-forming plants, the regenerated roots of which have been excised, placed in an upright position in vermiculite before being inoculated with R. leguminosarum 3 days later.

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