Figure 4
From: An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants
The effect of ethanol on rates of AL-PCD in Arabidopsis thaliana. Seven day old cell suspension cultures (A) and 5 day old seedlings (B) of Arabidopsis thaliana were treated with 10% ethanol and then scored for AL-PCD 24 hr (cells) or 6 hr (root hairs) later. Cells/root hairs showing retraction and condensation of the cytoplasm and no FDA staining were deemed to have undergone AL-PCD. Those root hairs/cells showing neither FDA staining nor retraction of the cytoplasm were scored as necrotic. Rates of AL-PCD or Total Cell Death (TCD) (TCD = Necrotic + AL-PCD) were plotted. Error bars = standard error of n = 3 replicates.