The effect of heat treatment on rates of AL-PCD in Arabidopsis thaliana. Rates of AL-PCD were determined in (A) Seven day old cell suspension cultures subjected to a range of temperatures for 10 min and scored 24 hr later and (B) root hairs subjected to a range of temperatures for ten minutes and scored 6 hr later. Cells/root hairs showing retraction and condensation of the cytoplasm and no FDA staining were deemed to have undergone AL-PCD. Those root hairs/cells showing neither FDA staining nor retraction of the cytoplasm were scored as necrotic. Rates of AL-PCD or Total Cell Death (TCD) (TCD = necrotic + AL-PCD) were plotted. Error bars = standard error of n = 3 replicates.