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Table 3 HRM genotyping results of F1 progeny of BraA.cax1.a and BraA.met1.a lines crossed to wild-type.

From: High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes

Linea

Plantb

Number of heterozygous plantsc

Number of wild-type plantsd

BraA.cax1.a-1

E

5

0

BraA.cax1.a-4

C

2

0

BraA.cax1.a-6

B

4

2

BraA.cax1.a-7

A

3

3

BraA.cax1.a-8

A

4

1

BraA.cax1.a-10

A

3

2

BraA.cax1.a-11

B

3

3

BraA.cax1.a-12

A

3

3

BraA.cax1.a-14

B

3

0

BraA.cax1.a-18

D

2

4

BraA.cax1.a-20

A

2

2

BraA.met1.a-6

A

5

5

  1. a - TILLING line crossed to B. rapa R-o-18 wild-type, b - Individual M3 plant identifier' c - Number of individual F1 heterozygous plants identified by HRM' d - Number of individual F1 wild-type plants identified by HRM