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Table 3 HRM genotyping results of F1 progeny of BraA.cax1.a and BraA.met1.a lines crossed to wild-type.

From: High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes

Linea Plantb Number of heterozygous plantsc Number of wild-type plantsd
BraA.cax1.a-1 E 5 0
BraA.cax1.a-4 C 2 0
BraA.cax1.a-6 B 4 2
BraA.cax1.a-7 A 3 3
BraA.cax1.a-8 A 4 1
BraA.cax1.a-10 A 3 2
BraA.cax1.a-11 B 3 3
BraA.cax1.a-12 A 3 3
BraA.cax1.a-14 B 3 0
BraA.cax1.a-18 D 2 4
BraA.cax1.a-20 A 2 2
BraA.met1.a-6 A 5 5
  1. a - TILLING line crossed to B. rapa R-o-18 wild-type, b - Individual M3 plant identifier' c - Number of individual F1 heterozygous plants identified by HRM' d - Number of individual F1 wild-type plants identified by HRM