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Table 2 Details of primers used for gene isolation and HRM analysis.

From: High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes

Primera

Geneb

Stagec

Mutation Targetedd

Sequence 5' to 3'e

BrCAX1 TILLING_Forward

BraA.CAX1.a

TILLING

 

AGAGATTTCCTAGCCATGTG

BrCAX1 TILLING_Reverse

BraA.CAX1.a

TILLING

 

CGACCCCTAATTGTTTTATGTG

BrCAX1 HRM1_Forward

BraA.CAX1.a

HRM

6,16

TCCTCGAAGTTGCCTCTGAT

BrCAX1 HRM1_Reverse

BraA.CAX1.a

HRM

6,16

GCTGCTGACCATTGTTCCTG

BrCAX1 HRM3_Forward

BraA.CAX1.a

HRM

7,8,12,14,20

GCAGGAACAATGGTCAGCAG

BrCAX1 HRM3_Reverse

BraA.CAX1.a

HRM

7,8,12,14,20

CGAGAATGACTTCTTGGAGATT

BrCAX1 HRM6_Forward

BraA.CAX1.a

HRM

4,10,11,18

TCCAGAAGGTTCCATACAAAGG

BrCAX1 HRM6_Reverse

BraA.CAX1.a

HRM

4,10,11,18

CGCTGTTCTGTTTAGTAATGTGTTG

BrMet1aF4

BraA.MET1.a

Gene isolation

 

ACTTCACAGCATCTCCTCAGGGC

BrMet1aF5

BraA.MET1.a

Gene isolation

 

CTTGACAATGGTGCTGTTATTCAG

BrMet1aF6

BraA.MET1.a

Gene isolation

 

GTCTGGTTTGATGGCAGAGGACG

BrMet1aR4

BraA.MET1.a

Gene isolation

 

CGCACTTCGCAGCTTCAGAAAC

BrMet1aR5

BraA.MET1.a

Gene isolation

 

AAATAGAGATTGTTGAACACCCCC

BrMet1aR6

BraA.MET1.a

Gene isolation

 

TTGGATACCACAGGTGCCATGC

BrMet1aHRMF

BraA.MET1.a

HRM

3,6,7,10,14

TGCGATGATAAGGAGAAAGG

BrMet1aHRMR

BraA.MET1.a

HRM

3,6,7,10,14

TTTGCCGTCTCATCCAAAC

  1. a- Primer name, b-gene primer designed to, c-stage of analysis primer used, d-line primers used to genotype, e- sequence of primer