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Table 1 Nucleotide changes in BraA.cax1.a and BraA.met1.a TILLING mutants.

From: High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes

Linea Mutation Changeb Position (base-pair)c Position (amino-acid)d Type of mutatione
BraA.cax1.a-1 G to A 881 - Non-coding
BraA.cax1.a-2 C to T 860 - Non-coding
BraA.cax1.a-3 G to A 545 2 Non-coding
BraA.cax1.a-4 G to A 771 77 Missense
BraA.cax1.a-5 C to T 494 - Non-coding
BraA.cax1.a-6 G to A 517 - Non-coding
BraA.cax1.a-7 G to A 673 44 Missense
BraA.cax1.a-8 G to A 599 19 Missense
BraA.cax1.a-9 G to A 645 35 Missense
BraA.cax1.a-10 C to T 736 65 Missense
BraA.cax1.a-11 G to A 795 85 Missense
BraA.cax1.a-12 C to T 708 56 Missense
BraA.cax1.a-13 C to T 689 49 Silent
BraA.cax1.a-14 C to T 661 40 Missense
BraA.cax1.a-15 C to T 658 39 Missense
BraA.cax1.a-16 C to T 421 - Non-coding
BraA.cax1.a-17 G to A 926 - Non-coding
BraA.cax1.a-18 C to T 818 92 Silent
BraA.cax1.a-19 C to T 732 64 Missense
BraA.cax1.a-20 C to T 690 50 Nonsense
BraA.met1.a-1 C to T 3516 248 Silent
BraA.met1.a-2 G to A 3334 188 Missense
BraA.met1.a-3 G to A 3334 188 Missense
BraA.met1.a-4 G to A 3084 104 Silent
BraA.met1.a-5 C to T 3081 103 Silent
BraA.met1.a-6 G to A 3252 243 Nonsense
BraA.met1.a-7 G to A 3500 243 Missense
BraA.met1.a-8 G to A 3012 80 Silent
BraA.met1.a-9 G to A 3495 241 Silent
BraA.met1.a-10 C to T 3335 188 Missense
BraA.met1.a-11 C to T 3069 99 Silent
BraA.met1.a-12 G to A 3330 186 Silent
BraA.met1.a-13 C to T 3324 184 Silent
BraA.met1.a-14 G to A 3213 147 Nonsense
BraA.met1.a-15 C to T 3265 165 Silent
BraA.met1.a-16 G to A 3330 186 Silent
BraA.met1.a-17 G to A 3258 162 Silent
  1. a - TILLING line, b - Nucleotide change in mutant line, c - Position of nucleotide change in base-pairs, d - Position of amino-acid change in peptide, e - Type of mutation caused by nucleotide change. Non-coding - nucleotide change not within coding region, missense - nucleotide change results in an amino-acid change, nonsense - nucleotide change results in the introduction of a premature stop codon, silent - no change in amino-acid.