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Figure 1 | Plant Methods

Figure 1

From: High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes

Figure 1

HRM analysis for Brassica rapa R-o-18 wild-type and individual plants from M 3 BraA.cax1.a-11 and BraA.met1.a-6 TILLING mutants. A: Normalised melt curves for fluorescent signals from DNA strand dissociation of triplicated technical replicates of B. rapa wild-type (light green lines) and individual plants of M3 TILLING mutant BraA.cax1.a-11, plant BraA.cax1.a-11A (red), plant BraA.cax1.a-11B (red), plant BraA.cax1.a-11C (blue), plant BraA.cax1.a-11D (light green), plant BraA.cax1.a-11E (dark green). B: Normalised melt curves for fluorescent signals from DNA strand dissociation of triplicated technical replicates of B. rapa wild type (red lines) and individual plants M3 TILLING mutant BraA.met1.a-6, plant BraA.met1.a-6-1 (light blue), plant BraA.met1.a-6-2 (green), plant BraA.met1.a-6-3 (orange), plant BraA.met1.a-6-4 (purple), plant BraA.met1.a-14-1 (blue). C: Difference plot of genotypes' fluorescence normalised to wild type samples (light green lines, i) and individual plants of M3 TILLING mutant BraA.cax1.a-11, plant BraA.cax1.a-11A (red, ii), plant BraA.cax1.a-11B (red, iii), plant BraA.cax1.a-11C (blue, iv), plant BraA.cax1.a-11D (light green, v), plant BraA.cax1.a-11E (dark green, vi). D: Difference plot of genotypes' fluorescence normalised to wild type (red, i) and individual plants of the M3 TILLING mutant BraA.met1.a-2, plant BraA.met1.a-6-1 (light blue, ii), plant BraA.met1.a-6-2 (green, iii), plant BraA.met1.a-6-3 (orange, iv), plant BraA.met1.a-6-4 (purple, v), plant BraA.met1.a-14-1 (blue, vi).

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