Skip to main content
Figure 4 | Plant Methods

Figure 4

From: A novel Gateway®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens

Figure 4

PCR analysis of recombinant clones of Agrobacterium tumefaciens. (A). Clones of A. tumefaciens transformed with pEG101-Luc. M: 1 kb marker; lanes 1 through 10 were from bacteria colonies carrying the recombinant plasmid pEG101-Luc, where a 1.4 kb band was amplified corresponding with the expected 35S-Luc gene; lane 11 was a negative PCR control using A. tumefaciens only as a template; lane 12 was a positive PCR control using plasmid DNA of pEG101-Luc isolated from E. coli as a template. (B). Clones of A. tumefaciens transformed with pEG101-Aae2166. M: 1 kb marker; lanes 1 to 10 were clones carrying the recombinant plasmid pEG101-Aae2166. Amplified fragments corresponded to 35S-Aae2166 (1.1 kb). Lane 11 was a negative PCR control using A. tumefaciens only as a template. Lane 12 was a positive control using plasmid DNA of pEG101-Aae2166 isolated from E. coli as a template.

Back to article page

Plant Methods

ISSN: 1746-4811