Figure 3From: A novel Gateway®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciensThe destination vector pEG101-SacB/R can be stably propagated in the E. coli strain DH5α. Restriction digestion (Bst XI and Bbv CI) of plasmid DNAs, isolated from five repeated overnight cultures of E. coli carrying the pEG101-SacB/R destination vector, showed identical restriction patterns. M: 1 kb marker. Lanes 1 through 5 were digested plasmid DNAs of five repeated subcultures.Back to article page