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Figure 1 | Plant Methods

Figure 1

From: A novel Gateway®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens

Figure 1

Schematic cloning of a gene into a binary expression vector and selection in Agrobacterium tumefaciens. (A) Key features of pEG101-SacB/R. LB: T-DNA left border; RB: T-DNA right border; CmR: chloramphenicol resistance gene; attL1 and attL2: recognition sites of the LR clonase; ccdB: the Invitrogen ccdB gene cassette frame B; SacB/SacR: Levansucrase genes along with their native promoter; Bbv CI and Bst XI: restriction enzyme sites for replacing the ccdB gene fragment with the SacB/SacR gene cassette. (B). Compared to the regular LR cloning procedure, the new protocol is more convenient and time-saving.

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Plant Methods

ISSN: 1746-4811