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Figure 4 | Plant Methods

Figure 4

From: Protocol: high throughput silica-based purification of RNA from Arabidopsis seedlings in a 96-well format

Figure 4

Assessment of quality of HTP purified RNA. The quality of the total RNA purified using the HTP RNA extraction procedure was analysed by UV spectrometry using a NanoDrop (A) and an Agilent Bioanalyzer (B, C) which demonstrated the consistent quality of the RNA. A: Measured A260/280 ratios for the four samples shown were 2.05, 2.07, 2.03 and 2.02 while the corresponding figures for the A260/230 ratios were 1.63, 1.39, 1.12 and 1.44. B: Representative electropherograms and C: corresponding digital electrophoresis gels images show clear sharp ribosomal RNA peaks/bands typical of high quality Arabidopsis RNA. In addition to the cytoplasmic 25S and 18S rRNA peaks, other peaks corresponding to 23S and 16S rRNA from chloroplasts and 5S and small rRNA are evident.

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