A simple and efficient method for isolating small RNAs from different plant species

Table 1 Concentration* of total RNA and LMW RNA samples obtained using different extraction methods

Method	Tissue	Average 260/280	Average 260/230	total RNA yield (μg)	260/280	260/230	LWM RNA yield (μg)
TRIzol^®	buds	1.84	0.21	76.3	1.96	1.73	56.34
	pads	1.83	0.25	50.28	1.96	1.59	38.4
LiCl	buds	2.19	2.03	34.18	1.85	2.25	16.33
	pads	1.76	1.72	4.20	1.85	2.24	1.57
LiCl supernant	buds	---	---	---	2.03	1.94	29.88
	pads	---	---	---	1.87	1.61	24.42
CTAB	buds	2.19	2.01	24.65	2.12	2.22	16.81
	pads	1.86	1.29	17.60	2.05	2.15	10.26

*Yields and spectrophotometric A260/A230 and A260/A280 ratios of total RNA and LMW RNA samples extracted from cactus floral buds and pads with different extraction methods. 1 g of tissue was used for each total RNA extraction (in triplicate) and quantified in a Nanodrop spectrophotometer, followed by LMW RNA extraction (from 3 pooled total RNA samples).

ISSN: 1746-4811