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Figure 4 | Plant Methods

Figure 4

From: A simple and efficient method for isolating small RNAs from different plant species

Figure 4

Visualization and northern blot assays of small RNAs isolated from different plant species. a) Gel electrophoresis of LMW RNA from cactus (floral buds), Arabidopsis (leaves), agave (leaf), banana (skin of the fruit), tobacco (leaf), and tomato (fruit) isolated (triplicate) with the optimized LiCl method (omitting the LiCl precipitation step; Figure 2-modification 4), b) Northern blot assay with de miRNA159a, and c) with the small nucleolar RNA U6 probe (same samples as in the gel presented in a)). 0.1 g of tissue was used for each sample extraction (in triplicate). 2 μg of each sample was loaded on a 12.5% polyacrylamide gel. M4, 10 bp DNA Ladder (Invitrogen); M5, microRNA Marker (NEB).

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