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Figure 1 | Plant Methods

Figure 1

From: A simple and efficient method for isolating small RNAs from different plant species

Figure 1

Comparison of total RNA and low-molecular-weight (LMW) RNA samples isolated from cactus ( Opuntia robusta ) with different extraction methods. a) Total RNA was extracted (in triplicate) with three different RNA extraction methods. 0.1 g of tissue was used for each sample and 1/10 volume of each sample was loaded on a 1% agarose gel (gel stained with ethidium bromide). b) LMW RNA extracted from total RNA samples obtained with different extraction methods, 1/10 volume (1-3 ug) of each sample was loaded on a 12.5% polyacrylamide gel (gel stained with SYBR gold). M1, 1 kb DNA ladder (Invitrogen); M2, Low Range ssRNA Ladder (NEB); and M3, oligonucleotide ladder.

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