Table 2 Analysis for PCR bias of different primer sets
From: Universal endogenous gene controls for bisulphite conversion in analysis of plant DNA methylation
Primer set | Percentage (%) of PCR products from genomic DNA in TapidorDH/Value of bias | Average value of bias | Percentage (%) of PCR products from genomic DNA in Nipponbare/Value of bias | Average value of bias | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Â | 80 a | 60 a | 40 a | 20 a | Â | 80 a | 60 a | 40 a | 20 a | Â |
ATP1-1 | 95/4.75 | 90/6.00 | 85/8.50 | 75/12.00 | 7.81 | 95/4.75 | 65/1.24 | 60/2.25 | 45/3.27 | 2.88 |
ATP1-2 | 90/2.25 | 80/2.67 | 65/2.79 | 30/1.71 | 2.36 | 85/1.42 | 70/1.56 | 60/2.25 | 40/2.67 | 1.98 |
IND.a_A3 | 95/4.75 | 85/3.78 | 85/8.50 | 40/2.67 | 4.93 | / | / | / | / | / |
NAD | 95/4.75 | 90/6.00 | 85/8.50 | 70/9.33 | 7.15 | / | / | / | / | / |