Table 1 Sodium bisulphite conversion frequencies of four treatments
From: Universal endogenous gene controls for bisulphite conversion in analysis of plant DNA methylation
Treatment | Number of clones | Conversion frequency (%) | ||||
|---|---|---|---|---|---|---|
| Â | Â | CG | CHG | CHH | Total | t test (P < 0.01) |
One round treatment | 15 | 97.57 | 98.79 | 98.18 | 98.18 | A |
One round treatment + purification | 15 | 96.36 | 96.97 | 98.99 | 98.06 | A |
Two round treatment | 15 | 99.80 | 99.39 | 99.80 | 99.64 | B |
Two round treatment + purification | 15 | 99.39 | 100.00 | 99.80 | 99.76 | B |