An efficient procedure for plant organellar genome assembly, based on whole genome data from the 454 GS FLX sequencing platform

Table 4 Analysis of minimal sequencing coverage for the complete organellar genome assembly of B. hygrometrica

Sample data (Mbp)	Sequencing coverage (X)*	cpDNA coverage (%)	mtDNA coverage (%)
50	0.17	47.32	9.05
100	0.33	88.08	43.77
200	0.67	98.90	88.72
400	1.33	100.00	99.82
600	2.00	100.00	100.00
800	2.67	100.00	100.00
1000	3.33	100.00	100.00
1200	4.00	100.00	100.00
1400	4.67	100.00	100.00

*The flow cytometry study showed that the genome size of B. hygrometrica is about twice that of Arabidopsis thaliana (~300 Mbp).

ISSN: 1746-4811