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Figure 1 | Plant Methods

Figure 1

From: Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

Figure 1

Effects of solvents on hormone extraction. Concentration of endogenous plant hormones (A) Z, DHZ, 2iP, IAA, ACC, and (B) ABA, JA, SA, GA4, GA9, GA24 detected in rosemary leaves using the following extraction solvents: M = methanol: glacial acetic acid, 99:1 (v/v); 80:20 M/I = methanol: isopropanol: glacial acetic acid 80:19:1 (v/v/v); 60:40 M/I = methanol: isopropanol: glacial acetic acid 60:39:1 (v/v/v); 40:60 M/I = methanol: isopropanol: glacial acetic acid 40:59:1 (v/v/v); 20:80 M/I = methanol: isopropanol: glacial acetic acid 20:79:1 (v/v/v); I = isopropanol: glacial acetic acid, 99:1 (v/v); 100:100 M/I = leaves were extracted first with methanol: glacial acetic acid, 99:1 (v/v) in two extraction steps and then with I = isopropanol: glacial acetic acid, 99:1 (v/v) in two extraction steps.

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Plant Methods

ISSN: 1746-4811