Figure 5

Regeneration of transgenic P. aegyptiaca by inoculation onto tomato host roots. White light (A, C, E, G, and I) and UV illumination (B, D, F, H, and J) of transgenic P. aegyptiaca. A and B) Explants 4 days after inoculation onto tomato roots. C and D) Eight days after inoculation P. aegyptiaca developed a haustorium and penetrated the tomato root. E and F) Twenty days after inoculation the parasite established full vascular connection with the tomato host, developing a tubercle near the point of haustorial attachment. The rest of inoculated parasitic explant tissue has died. G and H) Twenty five days after inoculation the P. aegyptiaca transgenic tubercle developed the spider-like stage. I and J) Thirty days after inoculation transgenic parasitic shoot buds regenerated. Ph, P. aegyptiaca; Tom, tomato; Ha, haustorium; B, shoot bud.