Figure 4
From: Transformation and regeneration of the holoparasitic plant Phelipanche aegyptiaca
Detection of YFP reporter gene by PCR and Southern blot hybridization. A) Amplification of mas-YFP sequence from genomic DNA of non transgenic (lanes 1-3) and transgenic (lanes 4-6) P. aegyptiaca cultures; lane 7, ntc; lane 8, plasmid pHG8-YFP. B) Amplification of 18S from the same non transgenic (lane 1-3) and transgenic (lane 4-6) genomic DNA used in panel A; lane 7, ntc; lane 8, plasmid pHG8-YFP. C) Southern blot hybridization of mas-YFP probe to plasmid pHG8-YFP (lane 1, 1 hour exposure). Genomic DNA of non transgenic P. aegyptiaca callus digested with EcoR1 (Lane 2), EcoRV (Lane 3), XbaI (Lane 4) and YFP- expressing P. aegyptiaca callus digested with EcoR1 (Lane 5), EcoRV (Lane 6), and XbaI (Lane 7). Image for lanes 2-7 was 9 days exposure after after removing lane 1.