Figure 1

P. aegyptiaca in vitro sterile conditions for growth and transformation. A) P. aegyptiaca radicle emerged from the seed coat 7 days after GR24 application. B) One-month-old P. aegyptiaca callus developed from seedling with seed coat still visible. C) Three-month-old P. aegyptiaca callus just prior to transformation. D) Fragments of the P. aegyptiaca callus chopped into multiple small explants and co-cultivated in the dark with A. rhizogenes on solid media. E) P. aegyptiaca explants returned to liquid media 5 days after transformation.