Figure 2
From: Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
Optimization of sonication conditions to yield ~200-bp chromatin fragments. 2 × 107 cells of Chlamydomonas strains cw15-325 and CRR16 were sonicated 20 times 20 sec. DNA was extracted, separated on a 1.5% agarose gel and stained with Ethidium bromide.