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Figure 2 | Plant Methods

Figure 2

From: A pair of new BAC and BIBAC vectors that facilitate BAC/BIBAC library construction and intact large genomic DNA insert exchange

Figure 2

DNA analyses of randomly picked colonies showing copy number increases of the composite vectors pHZAUBAC1 and pHZAUBIBAC1. A. DNA analysis of randomly picked colonies transformed with pIndigoBac536 (lane 10), pIndigoBac536-S (lane 9) and the ligation product of pIndigoBac536-S and pGEM-4Z at the Hin dIII cloning site (pHZAUBAC1, lanes 1-8). DNA samples were prepared from 2 mL overnight cultures and were resuspended in 50 μL TE. Aliquots of 5 μL were digested with Hin dIII and were loaded onto a 1% agarose gel. pIndigoBAC536-S is 470 bp smaller than pIndigoBAC536. B. DNA analysis of randomly picked colonies transformed with BIBAC-S (lane 9) and the ligation product of BIBAC-S and pGEM-4Z at the Bam HI cloning site (pHZAUBIBAC1, lanes 1-8). DNA samples were prepared from 2 mL overnight cultures and were resuspended in 50 μL of TE. Aliquots of 5 μL were digested with Bam HI and were loaded onto a 1% agarose gel. The marker is lambda/Hin dIII.

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