Figure 4
Analysis of effector-triggered immunity using seedling-flood inoculation assays at high (5 × 106 CFU/ml) and low (1 × 105 CFU/ml) bacterial inoculums. A. Response of Arabidopsis (Col-0) seedlings flood-inoculated with a bacterial suspension (5 × 106 CFU/ml) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and Pst DC3000 carrying AvrRpt2 (Pst DC3000 AvrRpt2) at 1 and 2 days post-inoculation. B. Response of Arabidopsis (Col-0) seedlings flood-inoculated with a low concentration (1 × 105 CFU/ml) bacterial suspension of Pst DC3000 and Pst DC3000 AvrRpt2. Photographs were taken at 4 dpi. C. Bacterial populations of Pst DC3000 and Pst DC3000 AvrRpt2 in Arabidopsis (Col-0) seedlings flood-inoculated with a low concentration (1 × 105 CFU/ml) of bacterial suspension. Bacterial populations were quantified at 4 dpi.