Figure 1

Strategy followed to assign genomic and functional annotations to the reporters on the Agilent-016047 maize microarray. Using BLASTN and exonerate software, the 42,034 60-mer reporters were matched to available EST sequences, the maize B73 RefGen v2 genome and the WGS predicted transcripts. BLASTN and exonerate results were filtered and compared to test agreement between EST, WGS transcript and gDNA hits. Based on the agreement analysis, one of six genomic annotation groups was assigned for each reporter. Functional annotations of reporters were based on the functional annotations of their corresponding WGS transcripts. The data has been made accessible from the Maize Microarray Annotation Database http://MaizeArrayAnnot.bi.up.ac.za/.