Expression levels of OsGLK1-upregulated genes in rice green tissue protoplasts under light and NF treatments. Protoplasts transfected with/without OsGLK1-GFP or GFP were treated with 40 μmol m-2 s-1 light and/or 500 nM NF for 12 h. A-D, Transcript levels of OsLhcb1, OsLhcp, GADPH and RbcS were detected by quantitative real-time PCR and normalized to that of β-actin. Values are means, with standard errors indicated by bars, representing 3 independent biological samples, each with 3 technical replicates. E, Detection of transiently expressed OsGLK1-GFP (lanes 1-2) and GFP (lanes 3-4) by Western blot. CK was a negative control. The upper panel shows an immunoblot using a monoclonal rabbit anti-GFP antibody. As a loading control, a Coomassie Brilliant Blue (CBB)-stained PVDF membrane is shown in the lower panel.