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Figure 4 | Plant Methods

Figure 4

From: A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes

Figure 4

Subcellular localization analysis in rice green tissue protoplasts. Rice proteins and Arabidopsis organelle markers were transiently expressed in rice green tissue protoplasts. A, Rice OsRpl6-2-YFP labeling of mitochondria. B, Rice OsTRX m5-GFP labeling of plastids. C, OsTRX m2-GFP targeted to chloroplasts. D, BAS1-GFP targeted to chloroplasts. E, CD3-998 labeling of plastids; CD3-982 labeling of peroxisomes; CD3-958 labeling of the endoplasmic reticulum (ER); CD3-966 labeling of Golgi. F, An mCherry-based plastid marker CD3-1000. Merged images are shown with YFP, mCherry or GFP and chlorophyll autofluorescence (Chl). Bright field images of protoplasts are also shown. Scale bars = 10 μm.

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