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Figure 3 | Plant Methods

Figure 3

From: A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes

Figure 3

Transient expression efficiencies of different sized plasmids in rice green tissue protoplasts. A-B, A 4.5 kb plasmid pUC-GFP (A) and a 13 kb plasmid CD3-998 (B) were transiently expressed in rice green tissue protoplasts. Merged images of GFP or YFP and chlorophyll autofluorescence (Chl) as well as bright field images of protoplasts are shown. Scale bars = 20 μm. C, Transfection efficiency of a 13 kb plasmid compared with that of a 4.5 kb plasmid, expressed as the ratio of GFP or YFP-positive cells to the total number of protoplasts (n ≥ 100). Values are means, with standard errors indicated by bars, representing at least 5 replicates.

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