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Figure 1 | Plant Methods

Figure 1

From: A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes

Figure 1

Isolation of protoplasts from rice green tissue. A, A representative healthy 8-day-old rice seedling used for protoplast isolation. Scale bar = 1 cm. B, Red markers indicate the optimal sections of seedlings (stem and sheath) yielding protoplasts. C, Cut strips were treated with 0.6 M mannitol followed by enzymatic digestion. D, Image of protoplasts obtained using a Nikon digital camera under an Olympus microscope with a 40× objective. Scale bar = 10 μm.

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