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Figure 1 | Plant Methods

Figure 1

From: Rapid analysis of seed size in Arabidopsis for mutant and QTL discovery

Figure 1

Reliable measurement of seed size is achieved using transmitted light. (A) Scanner with slide-holder insert allows simultaneous scanning of 24 samples. (B) Images are generated by emitting light from the lid, through the frame and onto the scanner bed; the seeds cast shadows which are detected by the scanner bed. (C) The resulting images are converted to solid black and white images using the threshold function of ImageJ prior to particle analysis. (D) Ten measurements were made on 54 seeds from a single silique, reorienting the seeds between measurements (circles = outliers > 1.5 IQR from median).

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