Table 3 Evaluation of hybridization signal intensities on PlasTi-microarrays
Experiment | A | B | C | D | E |
|---|---|---|---|---|---|
Overall spot intensity characteristics | |||||
A mean | 9.509 | 10.164 | 10.819 | 10.122 | 10.269 |
Norm. min (A min /A mean ) | 0.508 | 0.797 | 0.784 | 0.814 | 0.770 |
Norm. max (A max /A mean ) | 1.535 | 1.462 | 1.362 | 1.444 | 1.401 |
"Coding" probes with A probe > A mean | 287 (91.11%) | 254 (80.63%) | 286 (90.79%) | 268 (85.08%) | 277 (87.94%) |
"Non-coding" probes with A probe > A mean | 267 (20.32%) | 226 (17.20%) | 225 (17.12%) | 203 (15.45%) | 245 (18.65%) |
Non-specific signal characteristics | |||||
Aneg mean | 5.540 | 8.276 | 8.571 | 8.317 | 7.989 |
BUFFER (Abuff mean /Aneg mean ) | 1.021 ± 0.160 | 0.999 ± 0.020 | 1.002 ± 0.027 | 1.002 ± 0.026 | 1.001 ± 0.034 |
EMPTY (Aempty mean /Aneg mean ) | 0.916 ± 0.043 | 0.993 ± 0.018 | 0.989 ± 0.013 | 0.990 ± 0.014 | 0.987 ± 0.010 |
ALIEN (Aalien mean /Aneg mean ) | 1.031 ± 0.155 | 1.000 ± 0.026 | 1.001 ± 0.027 | 1.001 ± 0.022 | 1.002 ± 0.029 |
Athr | 7.288 | 8.862 | 9.125 | 8.799 | 8.637 |
Probes with A probe > Athr | 1094 (67.16%) | 1298 (79.68%) | 1244 (76.36%) | 1250 (76.73%) | 1225 (75.20%) |