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Figure 3 | Plant Methods

Figure 3

From: Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana

Figure 3

PHOT1-GFP and CLC-GFP fluorescence observations by VAEM and TIRFM. Still images taken from time-lapse sequences indicate localization of PHOT1-GFP (A and C) and CLC-GFP (B and D) in hypocotyl epidermal cells of 4-day-old Arabidopsis seedlings. Observations were made using VAEM (A, B) or TIRFM (C, D) observations. GFP-labeled pits in lateral-movement mode are indicated by arrows, and those in blinking mode are indicated by arrowheads. The shaded wedge in (B) indicates the trend of changing depth of VAE field illumination. TIRFM: total internal reflection fluorescence microscopy; VAEM: variable-angle epifluorescence microscopy. Bar = 20 μm.

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Plant Methods

ISSN: 1746-4811