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Table 5 Isolated transformants.

From: Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants

Interrupted gene ID1 Library Localization and orientation of the insertion2 Deletion size at the genomic region (bp) Expression of the interrupted gene
Sulfur starvation responsive genes
RDP3 183511 1 5'UTR, + n.a. n.a.
AOT4 206110 1 1st exon, + 0 truncated and co-expressed with the marker as a chimeric mRNA
TAUD1 127464 1 2nd intron, + 0 truncated and co-expressed with the marker as a chimeric mRNA. Low expression levels relative to the parental strain
TAUD2 77600 1 3rd exon, - n.a. mRNA not detected
HAP3 182794 1 4th exon,+ n.a. n.a.
PWR1 205514 1 1st exon, + 0 truncated and co-expressed with the marker as a chimeric mRNA.
Sulfate transporter genes
SULTR1 (1) 420357 1 3'UTR, + 8 n.a.
SULTR1 (2) 420357 1 promoter, + 3 n.a.
SULTR1 (3) 420357 1 17th exon, + 0 truncated and co-expressed with the marker as a chimeric mRNA
SULTR2 150514 1 9th exon, - 0 truncated and co-expressed with the marker as chimeric mRNA; transcript has a premature stop codon after splicing
SLT1 (1) 205502 1 8th exon, + 0 truncated and co-expressed with the marker as chimeric mRNA
SLT1 (2) 205502 2 promoter, + 12,000 n.a.
SLT2 205501 1 5'UTR, + 0 low expression levels relative to the parental strain
Hydrogen production and fermentation related genes
PFL1 (1) 206677 1 7th exon, + 0 truncated and co-expressed with the marker as a chimeric mRNA
PFL1 (2) 206677 1 1st intron, - 12 low expression levels relative to the parental strain
PFL1(3) 206677 1 5'UTR, - 0 low expression levels relative to the parental strain
FMR 145357 1 5'UTR, + n.a. n.a.
MME4 196831 1 6th exon, + 0 n.a.
HYDA1 (1) 183963 1 5'UTR, + n.a. n.a.
HYDA1 (2) 183963 1 3'UTR, - 2 n.a.
HYDA2 24189 1 9th exon, + 0 n.a.
PDC1 193810 1 5th introns, - n.a n.a
SEP (1) 147682 1 4th exon, + 0 n.a.
SEP (2) 147682 1 7th exon, + 4 n.a.
NADTH (1) 139758 1 3'UTR, - 0 n.a.
NADTH (2) 139758 1 3'UTR, - n.a. n.a.
PDK1 196270 1 3rd intron, + 0 n.a.
H4 163170 1 5'UTR, - n.a. n.a.
FDX 188740 1 2nd exon, + 15 n.a.
P4H-2 206683 1 3rd exon, + 0 n.a.
P4H-10 111255 1 7th exon, + 7 n.a.
P4H-10b 182877 1 3'UTR, - 0 n.a.
HCP4 148255 1 3'UTR, - 34 n.a.
ADH1 536345 1 15th exon, - 0 no protein detected
Other processes
RelA/SpoT homolog (1) 419232 1 promoter, + 0 n.a.
RelA/SpoT homolog (2) 419232 1 promoter, + 0 n.a.
AMT1;1 158745 2 3'UTR, - n.a. n.a.
CLPD 195417 1 3'UTR, + n.a. n.a.
PK2 174928 1 16th exon, + n.a. n.a.
WD 524057 1 17th exon, + 0 n.a.
GIP1 34332 1 2nd exon, + 0 n.a.
ABC1 523148 1 3'UTR, + n.a. n.a.
ABC2 60710 1 23rd exon, + n.a. n.a.
GOX8 196818 1 2nd exon, - n.a. n.a.
GOX15 196829 1 16th exon, + 0 n.a.
ATPASE 190023 1 3'UTR, - 1 n.a.
KIN 140337 1 5'UTR, + n.a. n.a.
  1. 1Protein IDs from the Joint Genome Institute Chlamydomonas v.4 website. 2Localization and orientation of the inserted marker gene relative to the interrupted target gene: + and - denote the same and opposite orientation, respectively. Independent insertion alleles for the same target gene are indicated by the same gene designation, but with a different number placed in a parenthesis. n.a.: not analyzed.