Table 5 Isolated transformants.

Sulfur starvation responsive genes

RDP3

183511

1

5'UTR, +

n.a.

n.a.

AOT4

206110

1

1^st exon, +

0

truncated and co-expressed with the marker as a chimeric mRNA

TAUD1

127464

1

2^nd intron, +

0

truncated and co-expressed with the marker as a chimeric mRNA. Low expression levels relative to the parental strain

TAUD2

77600

1

3^rd exon, -

n.a.

mRNA not detected

HAP3

182794

1

4^th exon,+

n.a.

n.a.

PWR1

205514

1

1^st exon, +

0

truncated and co-expressed with the marker as a chimeric mRNA.

Sulfate transporter genes

SULTR1 (1)

420357

1

3'UTR, +

8

n.a.

SULTR1 (2)

420357

1

promoter, +

3

n.a.

SULTR1 (3)

420357

1

17^th exon, +

0

truncated and co-expressed with the marker as a chimeric mRNA

SULTR2

150514

1

9^th exon, -

0

truncated and co-expressed with the marker as chimeric mRNA; transcript has a premature stop codon after splicing

SLT1 (1)

205502

1

8^th exon, +

0

truncated and co-expressed with the marker as chimeric mRNA

SLT1 (2)

205502

2

promoter, +

12,000

n.a.

SLT2

205501

1

5'UTR, +

0

low expression levels relative to the parental strain

Hydrogen production and fermentation related genes

PFL1 (1)

206677

1

7^th exon, +

0

truncated and co-expressed with the marker as a chimeric mRNA

PFL1 (2)

206677

1

1^st intron, -

12

low expression levels relative to the parental strain

PFL1(3)

206677

1

5'UTR, -

0

low expression levels relative to the parental strain

FMR

145357

1

5'UTR, +

n.a.

n.a.

MME4

196831

1

6^th exon, +

0

n.a.

HYDA1 (1)

183963

1

5'UTR, +

n.a.

n.a.

HYDA1 (2)

183963

1

3'UTR, -

2

n.a.

HYDA2

24189

1

9^th exon, +

0

n.a.

PDC1

193810

1

5^th introns, -

n.a

n.a

SEP (1)

147682

1

4^th exon, +

0

n.a.

SEP (2)

147682

1

7^th exon, +

4

n.a.

NADTH (1)

139758

1

3'UTR, -

0

n.a.

NADTH (2)

139758

1

3'UTR, -

n.a.

n.a.

PDK1

196270

1

3^rd intron, +

0

n.a.

H4

163170

1

5'UTR, -

n.a.

n.a.

FDX

188740

1

2^nd exon, +

15

n.a.

P4H-2

206683

1

3^rd exon, +

0

n.a.

P4H-10

111255

1

7^th exon, +

7

n.a.

P4H-10b

182877

1

3'UTR, -

0

n.a.

HCP4

148255

1

3'UTR, -

34

n.a.

ADH1

536345

1

15^th exon, -

0

no protein detected

Other processes

RelA/SpoT homolog (1)

419232

1

promoter, +

0

n.a.

RelA/SpoT homolog (2)

419232

1

promoter, +

0

n.a.

AMT1;1

158745

2

3'UTR, -

n.a.

n.a.

CLPD

195417

1

3'UTR, +

n.a.

n.a.

PK2

174928

1

16^th exon, +

n.a.

n.a.

WD

524057

1

17^th exon, +

0

n.a.

GIP1

34332

1

2^nd exon, +

0

n.a.

ABC1

523148

1

3'UTR, +

n.a.

n.a.

ABC2

60710

1

23^rd exon, +

n.a.

n.a.

GOX8

196818

1

2^nd exon, -

n.a.

n.a.

GOX15

196829

1

16^th exon, +

0

n.a.

ATPASE

190023

1

3'UTR, -

1

n.a.

KIN

140337

1

5'UTR, +

n.a.

n.a.