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Table 4 Sizes of genomic deletions generated by insertion of the marker gene.

From: Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants

 

List of deletions sizes* (bp)

n

Library 1

0 (×23), 2, 3, 4, 5 (×2), 8, 12 15, 34

32

Library 2

0 (×3), 12, 22, 200, 4178, 5000, 10000, 12000, 35000

11

  1. n, number of transformants analyzed. x, the number of times that the specified deletion size was found. 0 denotes that the insertion was 'true' with no deletion at either flank. * some deletion size analyses were noted for transformants isolated by forward genetic screens not described in this manuscript [36–38].
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Plant Methods

ISSN: 1746-4811