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Table 1 Main features of transformant libraries used for identification of strains harboring specific gene disruptions

From: Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants

  Number of transformants generated Marker used for transformation Transformation method Indexing method Preservation method Reference
Library 1 ~100,000 AphVIII
(1.7 kb PCR-fragment)
electroporation individuals none
(one-use library)
[35]; this work
Library 2 ~22,000 AphVIII
(4.7 kb linearized plasmid)
glass beads pools of 96 cryopreservation [2]