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Table 1 Main features of transformant libraries used for identification of strains harboring specific gene disruptions

From: Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants

 

Number of transformants generated

Marker used for transformation

Transformation method

Indexing method

Preservation method

Reference

Library 1

~100,000

AphVIII

(1.7 kb PCR-fragment)

electroporation

individuals

none

(one-use library)

[35]; this work

Library 2

~22,000

AphVIII

(4.7 kb linearized plasmid)

glass beads

pools of 96

cryopreservation

[2]