From: Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants
Number of transformants generated | Marker used for transformation | Transformation method | Indexing method | Preservation method | Reference | |
---|---|---|---|---|---|---|
Library 1 | ~100,000 | AphVIII (1.7 kb PCR-fragment) | electroporation | individuals | none (one-use library) | [35]; this work |
Library 2 | ~22,000 | AphVIII (4.7 kb linearized plasmid) | glass beads | pools of 96 | cryopreservation | [2] |